helmholtzeyeinstituteРоссийский офтальмологический журналRussian Ophthalmological Journal2072-00762587-5760Real time Publishers10.21516/2072-0076-2022-15-3-99-111helmholtzeyeinstitute-1057Research ArticleЭКСПЕРИМЕНТАЛЬНО-ЛАБОРАТОРНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL AND LABORATORY STUDIESВоздействие фрактальной зрительной стимуляции на здоровую сетчатку кролика: функциональные, морфометрические и биохимические исследованияImpact of fractal visual stimulation on healthy rabbit retina: functional, morphometric and biochemical studieshttps://orcid.org/0000-0002-8480-0894НероевВ. В.NeroevV. V.

Владимир Владимирович Нероев — академик РАН, профессор, доктор медицинских наук, начальник отдела патологии сетчатки и зрительного нерва, директор.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Vladimir V. Neroev — academician of the RAS, Dr. of Med. Sci., professor, head of the department of pathology of the retina and optic nerve, director.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

https://orcid.org/0000-0002-0161-5010ЗуеваМ. В.ZuevaM. V.

Марина Владимировна Зуева — доктор биологических наук, профессор, начальник отдела клинической физиологии зрения им. С.В. Кравкова.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Marina V. Zueva — Dr. Biol. Sci., professor, head of the department of clinical physiology of vision named after S.V. Kravkov.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

visionlab@yandex.ruhttps://orcid.org/0000-0003-1038-2746НероеваН. В.NeroevaN. V.

Наталия Владимировна Нероева — кандидат медицинских наук, врач отдела патологии сетчатки.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Nataliya V. Neroeva — Cand. of Med. Sci., ophthalmologist, ORCID: 0000-0003-1038-2746.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

https://orcid.org/0000-0003-1858-2005ФадеевД. В.FadeevD. V.

Денис Владимирович Фадеев — научный сотрудник научного экспериментальной центра.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Denis V. Fadeev — researcher, scientific experimental center.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

https://orcid.org/0000-0002-0148-8517ЦапенкоИ. В.TsapenkoI. V.

Ирина Владимировна Цапенко — кандидат биологических наук, старший научный сотрудник отдела клинической физиологии зрения им. С.В. Кравкова.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Irina V. Tsapenko — Cand. of Biol. Sci., senior researcher, head of the department of clinical physiology of vision named after S.V. Kravkov.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

https://orcid.org/0000-0003-1121-4314ОхоцимскаяТ. Д.OkhotsimskayaT. D.

Татьяна Дмитриевна Охоцимская — кандидат медицинских наук, врачофтальмолог отделения патологии сетчатки.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Tatiana D. Okhotsimskaya — Cand. of Med. Sci., оphthalmologist, department of pathology of the retina and optic nerve.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

https://orcid.org/0000-0003-4675-9648КотелинВ. И.KotelinV. I.

Владислав Игоревич Котелин — научный сотрудник отдела клинической физиологии зрения им. С.В. Кравкова.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Vladislav I. Kotelin — researcher, department of clinical physiology of vision named after S.V. Kravkov.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

https://orcid.org/0000-0001-8032-4248ПавленкоТ. А.PavlenkoT. A.

Татьяна Аркадьевна Павленко — кандидат медицинских наук, руководитель отдела патофизиологии и биохимии, клинико-диагностической лаборатории.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Tatiana A. Pavlenko — Cand. of Med. Sci., head of the department of pathophysiology and biochemistry, clinical diagnostic laboratory.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

https://orcid.org/0000-0002-7856-8005ЧесноковаН. Б.ChesnokovaN. B.

Наталья Борисовна Чеснокова — доктор биологических наук, профессор, главный специалист отдела патофизиологии и биохимии.

Ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Natalya B. Chesnokova — Dr. of Biol. Sci., professor, chief specialist, department of pathophysiology and biochemistry.

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

ФГБУ «НМИЦ глазных болезней им. Гельмгольца» Минздрава РоссииРоссияHelmholtz National Medical Research Center of Eye DiseasesRussian Federation20222309202215399111Copyright © Нероев В.В., Зуева М.В., Нероева Н.В., Фадеев Д.В., Цапенко И.В., Охоцимская Т.Д., Котелин В.И., Павленко Т.А., Чеснокова Н.Б., 20222022Нероев В.В., Зуева М.В., Нероева Н.В., Фадеев Д.В., Цапенко И.В., Охоцимская Т.Д., Котелин В.И., Павленко Т.А., Чеснокова Н.Б.Neroev V.V., Zueva M.V., Neroeva N.V., Fadeev D.V., Tsapenko I.V., Okhotsimskaya T.D., Kotelin V.I., Pavlenko T.A., Chesnokova N.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://roj.igb.ru/jour/article/view/1057

Цель работы — изучить динамику электрофизиологической активности сетчатки здоровых кроликов при различной длительности курсов фрактальной стимуляции (ФС) для получения новых научных данных о характере воздействия на сетчатку низкоинтенсивных зрительных фрактальных сигналов, самоподобных во временном масштабе.

Материал и методы

Материал и методы. Двенадцать здоровых кроликов (24 глаза) породы шиншилла обследованы до и после курсов ФС, длящихся 1, 4 и 12 нед, с помощью электроретинографических (ЭРГ), морфометрических (оптической когерентной томографии) и биохимических (определение дофамина в слезе) методов. Для ФС кроликов разработан прибор со светодиодным излучателем, формирующим нелинейную флуктуацию яркости на основе фрактальных функций Вейерштрасса — Мандельброта. Обоснован выбор используемых в работе параметров фрактального сигнала. Регистрировали паттерн-ЭРГ и ганцфельд-ЭРГ по стандартам ISCEV и фотопическую ритмическую ЭРГ на 8,3, 10, 12 и 24 Гц.

Результаты

Результаты. Отрицательного воздействия 12-недельного курса ФС на активность и морфологию сетчатки и на дофаминергические процессы в глазу здорового животного не обнаружено, что говорит о безопасности применения низкоинтенсивной ФС в клинике. Отмечено значимое увеличение амплитуды низкочастотных ритмических ЭРГ, укорочение пиковой латентности и возрастание амплитуды b-волны скотопической и фотопической ЭРГ.

Заключение

Заключение. Для оценки возможных терапевтических эффектов ФС необходимо продолжение исследований на животных моделях и у пациентов с патологией сетчатки. С учетом динамики активности сетчатки в данной работе в дальнейших исследованиях рекомендована продолжительность курсов ФС от недели до месяца.

Purpose

Purpose: to investigate the changes of electrophysiological activity of healthy rabbit retina occurring in courses of fractal stimulation (FS) of varied duration in order to obtain new scientific data on how fractal visual signals of low intensity, self-similar with respect to time, affect the retina.

Material and methods

Material and methods. 12 healthy Chinchilla rabbits (24 eyes), were examined before and after FS courses that lasted 1, 4 or 12 weeks, using electroretinographic (ERG), morphometric (optical coherence tomography) and biochemical methods (detection of dopamine in the tear). For FS of rabbits, a device with an LED emitter was developed, which generates nonlinear brightness fluctuation based on the Weierstrass — Mandelbrot fractal functions. The choice of fractal signal parameters used in the work was substantiated. Pattern ERG and ganzfeld ERG were registered according to ISCEV standards; also, photopic flicker ERG was recorded at 8.3, 10, 12, and 24 Hz.

Results

Results. No negative effects of a 12-month FS course on the activity and morphology of the retina, or on dopaminergic processes in the eye of a healthy animal were found, which confirms the safety of using low-intensity FS in the clinic. A statistically significant increase in the amplitude of low-frequency flicker ERGs, a shortening of peak latency, and an increase in the amplitude of the b-wave of the scotopic and photopic ERGs was noted.

Conclusion

Conclusion. To assess possible therapeutic effects of FS, we need to continue the investigation on animal models and human patients with retinal pathology. Considering the changes of retinal activity as revealed in the present paper, we recommend the duration of FS courses from 1 week to 1 month for future studies.

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