helmholtzeyeinstituteРоссийский офтальмологический журналRussian Ophthalmological Journal2072-00762587-5760Real time Publishers10.21516/2072-0076-2020-13-4-39-47helmholtzeyeinstitute-518Research ArticleЭКСПЕРИМЕНТАЛЬНО-ЛАБОРАТОРНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL AND LABORATORY STUDIESЭкспериментальное изучение влияния ресвератрола на нейротрофические и структурные изменения тканей при ретинальной ишемииAn experimental study of resveratrol effect on neurotrophic and structural changes in retinal ischemiaКиселеваТ. Н.KiselevaT. N.

Татьяна Николаевна Киселева — д-р мед. наук, профессор, начальник отдела ультразвуковых исследований глаза

ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Tatyana N. Kiseleva — Dr. of Med. Sci., professor, head of ultrasound diagnostic department

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

ЧудинА. В.ChudinA. V.

Антон Вячеславович Чудин — канд. мед. наук, заведующий офтальмологическим отделением

ул. Республики, д. 78, г. Ишим, 627750

Anton V. Chudin — Cand. of Med. Sci., head of ophthalmology department

78, Respubliki St., Ishim, 627750

БалацкаяН. В.BalatskayaN. V.

Наталья Владимировна Балацкая — канд. мед. наук, начальник отдела иммунологии и вирусологии

ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Natalya V. Balatskaja — Cand. of Med. Sci., head of the department of immunology and virology

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

ЩипановаА. И.ShchipanovaA. I.

Александра Ивановна Щипанова — канд. биол. наук, начальник экспериментального научного центра

ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Aleksandra I. Shchipanova — Cand. of Biol. Sci., head of the experimental scientific center

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

Хорошилова-МасловаИ. П.Khoroshilova-MaslovaI. P.

Инна Петровна Хорошилова-Маслова — д-р мед. наук, профессор, начальник отдела патологической анатомии и гистологии

ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Inna P. Khoroshilova-Maslova — Dr. of Med. Sci., professor, head of the department of pathological anatomy and histology

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

ЗайцевМ. С.ZaytsevM. S.

Максим Сергеевич Зайцев — младший научный сотрудник отдела ультразвуковых исследований глаза

ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Maksim S. Zaytsev — junior researcher of ultrasound diagnostic department

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

zaicev1549@yandex.ruМайбогинА. М.MayboginA. M.

Артемий Михайлович Майбогин — врач-патологоанатом отдела патологической анатомии и гистологии

ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Artemiy M. Maybogin — pathologist of department of pathological anatomy and histology

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

ЛуговкинаК. В.LugovkinaK. V.

Ксения Вадимовна Луговкина — канд. мед. наук, научный сотрудник отдела ультразвуковых исследований глаза

ул. Садовая-Черногрязская, д. 14/19, Москва, 105062

Kseniya V. Lugovkina — Cand. of Med. Sci., researcher of ultrasound diagnostic department

14/19, Sadovaya-Chernogryazskaya St., Moscow, 105062

ФГБУ «НМИЦ глазных болезней им. Гельмгольца» Минздрава РоссииРоссияHelmholtz National Medical Research Center of Eye DiseasesRussian FederationГБУ МЗ Тюменской области «Областная больница № 4»РоссияRegional hospital No 4 of Tyumen regionRussian Federation2020161220201343947Copyright &#x00A9; Киселева Т.Н., Чудин А.В., Балацкая Н.В., Щипанова А.И., Хорошилова-Маслова И.П., Зайцев М.С., Майбогин А.М., Луговкина К.В., 20202020Киселева Т.Н., Чудин А.В., Балацкая Н.В., Щипанова А.И., Хорошилова-Маслова И.П., Зайцев М.С., Майбогин А.М., Луговкина К.В.Kiseleva T.N., Chudin A.V., Balatskaya N.V., Shchipanova A.I., Khoroshilova-Maslova I.P., Zaytsev M.S., Maybogin A.M., Lugovkina K.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://roj.igb.ru/jour/article/view/518ресвератролишемия-реперфузиясетчаткаапоптознейропротекторные свойстваresveratrolischemia/reperfusionretinaapoptosisneuroprotective propertiesReferencesКиселева Т.Н., Чудин А.В. Экспериментальное моделирование ишемического поражения глаза. Вестник РАМН. 2014; (11–12): 97–103.Kiseleva T.N., Chudin А.V. Experimental modeling of ischemic injury of the eye. Vestnik RАMN. 2014; (11–12): 97–103 (In Russian).Janáky M., Grósz A., Tóth E., Benedek K., Benedek G. Hypobaric hypoxia reduces the amplitude of oscillatory potentials in the human. ERG Documenta Ophthalmologica. 2007; 114 (1): 45–51. doi: 10.1007/s10633-006-9038-5Janáky M., Grósz A., Tóth E., Benedek K., Benedek G. Hypobaric hypoxia reduces the amplitude of oscillatory potentials in the human. ERG Documenta Ophthalmologica. 2007; 114 (1): 45–51. doi: 10.1007/s10633-006-9038-5Oku H., Fukuhara M., Kurimoto T., et al. Endothelin-1 (ET-1) is increased in rat retina after crushing optic nerve. Current Eye Research. 2008; 33 (7): 611–20. https://doi.org/10.1080/02713680802213614Oku H., Fukuhara M., Kurimoto T., et al. Endothelin-1 (ET-1) is increased in rat retina after crushing optic nerve. Current Eye Research. 2008; 33 (7): 611–20. https://doi.org/10.1080/02713680802213614Tinjust D., Kergoat H., Lovasik J. Neuroretinal function during mild systemic hypoxia. Aviation, Space, and Environmental Medicine. 2002; 73 (12): 1189–94. PMID: 12498547Tinjust D., Kergoat H., Lovasik J. Neuroretinal function during mild systemic hypoxia. Aviation, Space, and Environmental Medicine. 2002; 73 (12): 1189–94. PMID: 12498547Carden D.L., Granger D.N. Pathophysiology of ischaemia-reperfusion injury. The journal of pathology. 2000 Feb; 90 (3): 255–66. doi: 10.1002/(SICI)10969896(200002)190:3<255::AID-PATH526>3.0.CO;2-6.Carden D.L., Granger D.N. Pathophysiology of ischaemia-reperfusion injury. The journal of pathology. 2000 Feb; 90 (3): 255–66. doi: 10.1002/(SICI)10969896(200002)190:3<255::AID-PATH526>3.0.CO;2-6.Гундорова Р.А., Швецова Н.Е., Иванов А.Н. и др. Модель ишемии сетчатки: клинико-функциональное и гистологическое исследование. Вестник офтальмологии. 2008; 73 (3): 18–23.Gundorova R.A., Shvecova N.E., Ivanov A.N., et al. Model of retinal ischemia: clinical-functional and histological examination. Vestnik oftal'mologii. 2008; 73 (3): 18–23 (In Russian).Каламкаров Г.Р., Цапенко И.В., Зуева М.В. и др. Экспериментальная модель острой ишемии сетчатки глаза у крыс. Бюллетень экспериментальной биологии и медицины. 2008; (6): 634–8.Kalamkarov G.R., Tsapenko I.V., Zueva M.V., et al. An experimental model of acute retinal ischemia in rats. Bjulleten' eksperimental'noj biologii i mediciny. 2008; 6: 634–8 (In Russian).Steele E. C., Guo Q., Namura S. Filamentous middle cerebral artery occlusion causes ischemic damage to retina in mice. Stroke. 2008; 39 (7); 2099–104. https://doi.org/10.1161/STROKEAHA.107.504357Steele E. C., Guo Q., Namura S. Filamentous middle cerebral artery occlusion causes ischemic damage to retina in mice. Stroke. 2008; 39 (7); 2099–104. https://doi.org/10.1161/STROKEAHA.107.504357Ueda K., Makahara T., Hoshino M., Mori A., Sakamoto K. Retinal blood vessels are damaged in rat model of NMDA-induced retinal degeneration. Neuroscience Letters. 2010; 485 (1): 55–9. https://doi.org/10.1016/j.neulet.2010.08.061Ueda K., Makahara T., Hoshino M., Mori A., Sakamoto K. Retinal blood vessels are damaged in rat model of NMDA-induced retinal degeneration. Neuroscience Letters. 2010; 485 (1): 55–9. https://doi.org/10.1016/j.neulet.2010.08.061Joachim S. C., Wax M. B., Boehm N., et al. Up-regulation of antibody response to heat shock proteins and tissue antigens in an ocular ischemia model. Invest. Ophthalmol. Vis. Sci. 2011 May; 52 (6): 3468–74. doi: 10.1167/iovs.10-5763Joachim S. C., Wax M. B., Boehm N., et al. Up-regulation of antibody response to heat shock proteins and tissue antigens in an ocular ischemia model. Invest. Ophthalmol. Vis. Sci. 2011 May; 52 (6): 3468–74. doi: 10.1167/iovs.10-5763Liu Y., Song X., Zhang D., et al. Blueberry anthocyanins: protection against ageing and light-induced damage in retinal pigment epithelial cells. British journal of nutrition. 2012 Jul; 108 (1): 16–27. doi: 10.1017/S000711451100523XLiu Y., Song X., Zhang D., et al. Blueberry anthocyanins: protection against ageing and light-induced damage in retinal pigment epithelial cells. British journal of nutrition. 2012 Jul; 108 (1): 16–27. doi: 10.1017/S000711451100523XКиселева Т.Н., Чудин А.В., Хорошилова-Маслова И.П. и др. Морфологические изменения в тканях сетчатки при регионарной ишемии-реперфузии в эксперименте in vivo. Бюллетень экспериментальной биологии и медицины. 2019; 167 (2): 250–7.Kiseleva T.N., Chudin A.V., Khoroshilova-Maslova I.P., et al. Morphological changes in retinal tissues during regional ischemia reperfusion in vivo in an experiment. Bjulleten' eksperemental'noj biologii i mediciny. 2019; 167 (2): 250–7 (In Russian).Li C., Wang L., Huang K., Zheng L. Endoplasmic reticulum stress in retinal vascular degeneration: protective role of resveratrol. Invest. Ophthalmol. Vis. Sci. 2012 May; 53 (6): 3241–9. doi: 10.1167/iovs.11-8406Li C., Wang L., Huang K., Zheng L. Endoplasmic reticulum stress in retinal vascular degeneration: protective role of resveratrol. Invest. Ophthalmol. Vis. Sci. 2012 May; 53 (6): 3241–9. doi: 10.1167/iovs.11-8406Vin A.P., Hu H., Zhai Y., et al. Neuroprotective effect of resveratrol prophylaxis on experimental retinal ischemic injury. Exp Eye Res. 2013 Mar; 108: 72–5. doi: 10.1016/j.exer.2012.11.022Vin A.P., Hu H., Zhai Y., et al. Neuroprotective effect of resveratrol prophylaxis on experimental retinal ischemic injury. Exp Eye Res. 2013 Mar; 108: 72–5. doi: 10.1016/j.exer.2012.11.022Liu X.Q., Wu B.J., Pan W.H., et al. Resveratrol mitigates rat retinal ischemic injury: the roles of matrix metalloproteinase-9, inducible nitric oxide, and heme oxygenase-1. J. Ocul. Pharmacol. Ther. 2013 Feb; 29 (1): 33–40. doi:10.1089/jop.2012.0141Liu X.Q., Wu B.J., Pan W.H., et al. Resveratrol mitigates rat retinal ischemic injury: the roles of matrix metalloproteinase-9, inducible nitric oxide, and heme oxygenase-1. J. Ocul. Pharmacol. Ther. 2013 Feb; 29 (1): 33–40. doi:10.1089/jop.2012.0141Huang W., Li G., Qiu J., Gonzalez P., Challa P. Protective effects of resveratrol in experimental retinal detachment. PLoS One. 2013 Sep; 8 (9): 725–35. doi: 10.1371/journal.pone.0075735Huang W., Li G., Qiu J., Gonzalez P., Challa P. Protective effects of resveratrol in experimental retinal detachment. PLoS One. 2013 Sep; 8 (9): 725–35. doi: 10.1371/journal.pone.0075735Kim Y.H., Kim Y.S., Kang S.S., Cho G.J., Choi W.S. Resveratrol inhibits neuronal apoptosis and elevated Ca2+ /calmodulin-dependent protein kinase II activity in diabetic mouse retina. Diabetes. 2010 Jul; 59 (7): 1825–35. doi: 10.2337/db09-1431Kim Y.H., Kim Y.S., Kang S.S., Cho G.J., Choi W.S. Resveratrol inhibits neuronal apoptosis and elevated Ca2+ /calmodulin-dependent protein kinase II activity in diabetic mouse retina. Diabetes. 2010 Jul; 59 (7): 1825–35. doi: 10.2337/db09-1431Luo H., Zhuang J., Hu P., et al. Resveratrol delays retinal ganglion cell loss and attenuates gliosis-related inflammation from ischemia-reperfusion injury Invest. Ophthalmol. Vis. Sci. 2018; 59 (10): 3879–38. https://doi.org/10.1167/iovs.18-23806Luo H., Zhuang J., Hu P., et al. Resveratrol delays retinal ganglion cell loss and attenuates gliosis-related inflammation from ischemia-reperfusion injury Invest. Ophthalmol. Vis. Sci. 2018; 59 (10): 3879–38. https://doi.org/10.1167/iovs.18-23806Luo J., He T., Yang J., et al. SIRT1 is required for the neuroprotection of resveratrol on retinal ganglion cells after retinal ischemia-reperfusion injury in mice. Graefes Arch. Clin. Exp. Ophthalmol. 2020; 258 (2): 335–44. https://doi.org/10.1007/s00417-019-04580Luo J., He T., Yang J., et al. SIRT1 is required for the neuroprotection of resveratrol on retinal ganglion cells after retinal ischemia-reperfusion injury in mice. Graefes Arch. Clin. Exp. Ophthalmol. 2020; 258 (2): 335–44. https://doi.org/10.1007/s00417-019-04580Seong H., Ryu J., Yoo W.S., Seo S.W. Resveratrol ameliorates retinal ischemia/ reperfusion injury in C57BL/6J mice via down regulation of Caspase-3. Current Eye Research. 2017; 42 (4): 1–9 https://doi.org/10.1080/02713683.2017.1344713Seong H., Ryu J., Yoo W.S., Seo S.W. Resveratrol ameliorates retinal ischemia/ reperfusion injury in C57BL/6J mice via down regulation of Caspase-3. Current Eye Research. 2017; 42 (4): 1–9 https://doi.org/10.1080/02713683.2017.1344713Cao R., Ishida T., Fang Y., et al. Protection of the retinal ganglion cells: intravitreal injection of Resveratrol in mouse model of ocular hypertension Invest. Ophthalmol. Vis. Sci. 2020 March; 61:13. https://doi.org/10.1167/iovs.61.3.13Cao R., Ishida T., Fang Y., et al. Protection of the retinal ganglion cells: intravitreal injection of Resveratrol in mouse model of ocular hypertension Invest. Ophthalmol. Vis. Sci. 2020 March; 61:13. https://doi.org/10.1167/iovs.61.3.13Pirhan D., Yuksel N., Emre E., Cengiz A., Kursat Yildiz D. Riluzole- and resveratrolinduced delay of retinal ganglion cell death in an experimental model of glaucoma. Curr. Eye Res. 2016; 41: 59–69. https://doi.org/10.3109/02713683.2015.1004719Pirhan D., Yuksel N., Emre E., Cengiz A., Kursat Yildiz D. Riluzole- and resveratrolinduced delay of retinal ganglion cell death in an experimental model of glaucoma. Curr. Eye Res. 2016; 41: 59–69. https://doi.org/10.3109/02713683.2015.1004719Statement for the Use of Animals in Ophthalmic and Vision Research.2016:10-35 Available at: http://www.arvo.org/About_ARVO/Policies/Statement_for_the_Use_of_Animals_in_Ophthalmic_and_Visual_Research/Statement for the Use of Animals in Ophthalmic and Vision Research.2016:10-35 Available at: http://www.arvo.org/About_ARVO/Policies/Statement_for_the_Use_of_Animals_in_Ophthalmic_and_Visual_Research/Рыбакова А.В., Макарова М.Н., Кухаренко А.Е., Вичаре А.С., Рюффер Ф.Р. Существующие требования и подходы к дозированию лекарственных средств лабораторным животным. Ведомости научного центра экспертизы средств медицинского применения. 2018; 8 (4): 207–17. https://doi.org/10.30895/19912919-2018-8-4-207-217Rybakova A.V., Makarova M.N., Kukharenko A.E., Vichare A.S., Rueffer F.R. Current Requirements for and Approaches to Dosing in Animal Studies. The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products. 2018; 8 (4): 207–17 (In Russian). https://doi.org/10.30895/1991-29192018-8-4-207-217Hua J., Guerin K. I., Chen J., et al. Resveratrol inhibits pathological retinal revascularization in Vldlr(-/-) mice. Invest. Ophthalmol. Vis. Sci. 2011; 52 (5): 2809–16.Hua J., Guerin K. I., Chen J., et al. Resveratrol inhibits pathological retinal revascularization in Vldlr(-/-) mice. Invest. Ophthalmol. Vis. Sci. 2011; 52 (5): 2809–16.Lau J., Dang M., Hockmann K., Ball A.K. Effects of acute delivery of endothelin-1 on retinal ganglion cell loss in the rat. Exp. Eye Res. 2006; 82 (1): 132–45. https://doi.org/10.1016/j.exer.2005.06.002Lau J., Dang M., Hockmann K., Ball A.K. Effects of acute delivery of endothelin-1 on retinal ganglion cell loss in the rat. Exp. Eye Res. 2006; 82 (1): 132–45. https://doi.org/10.1016/j.exer.2005.06.002

The authors declare that there are no conflicts of interest present.