The investigation of the association of alleles and genotypes of the polymorphic marker T786C of the eNOS gene at various stages of primary open-angle glaucoma
https://doi.org/10.21516/2072-0076-2024-17-1-28-31
Abstract
Purpose: to study the association of the polymorphic marker T786C of the eNOS gene with various stages of primary open-angle glaucoma (POAG).
Materials and methods. Peripheral blood of 90 patients aged 56 to 89 yrs (ave. 71 yrs) with POAG stages I, II, III was tested. DNA was isolated using a set of ribosorbents (Synthol company); subsequently, a real-time PCR reaction was performed on a DT-96 amplifier using the set to determine the polymorphic marker T786C in the eNOS gene.
Results. For all groups of patients, the frequency of occurrence of alleles and genotypes was calculated, and the relationship between the emergence of POAG and the presence of an unfavorable polymorphic marker was determined. The main changes were revealed at POAG stages II and III, whilst at stage I no effect of polymorphism T786C of the eNOS gene was observed.
Conclusion. For the first time, a comparative assessment was made of the distribution of alleles and genotypes by the polymorphic marker T786C in the eNOS gene of patients with various POAG stages.
About the Authors
T. V. Gavrilova
Academician E.A. Vagner Perm State Medical University
Russian Federation
Russian Federation
Tatyana V. Gavrilova — Corresponding member of the Russian Academy of Sciences, Dr. of Med. Sci., professor, head of chair of ophthalmology
26, Petropavlovskaya St., Perm, 614990
A. R. Kinkulkina
I. Sechenov First Moscow State Medical University; Mechnikov Research Institute for Vaccines and Sera
Russian Federation
Russian Federation
Aliya R. Kinkulkina — PhD student of the department microbiology, virology and immunology, F. Erisman Institute of Public Health, Sechenov First Moscow State Medical University; junior researcher of laboratories of molecular immunology, Mechnikov Research Institute for Vaccines and Sera
8, Bldg. 2, Trubetskaya St., Moscow, 119048
5A, Bldg. 1, Malyj Kazennyj per., Moscow, 105064
A. S. Avagyan
I. Sechenov First Moscow State Medical University; Mechnikov Research Institute for Vaccines and Sera
Russian Federation
Russian Federation
Asmik S. Avagyan — student, I. Sechenov First Moscow State Medical University; laboratory assistant, Mechnikov Research Institute for Vaccines and Sera
8, Bldg. 2, Trubetskaya St., Moscow, 119048
5A, Bldg. 1, Malyj Kazennyj per., Moscow, 105064
O. A. Svitich
I. Sechenov First Moscow State Medical University; Mechnikov Research Institute for Vaccines and Sera
Russian Federation
Russian Federation
Oksana A. Svitich — Corresponding member of the Russian Academy of Sciences, Dr. of Med. Sci., professor of the department of microbiology, virology and immunology, F. Erisman Institute of Public Health, I. Sechenov First Moscow State Medical University; director, Mechnikov Research Institute for Vaccines and Sera
8, Bldg. 2, Trubetskaya St., Moscow, 119048
5A, Bldg. 1, Malyj Kazennyj per., Moscow, 105064
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Review
For citations:
Gavrilova T.V., Kinkulkina A.R., Avagyan A.S., Svitich O.A. The investigation of the association of alleles and genotypes of the polymorphic marker T786C of the eNOS gene at various stages of primary open-angle glaucoma. Russian Ophthalmological Journal. 2024;17(1):28-31. (In Russ.) https://doi.org/10.21516/2072-0076-2024-17-1-28-31
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