The use of a combination of mesh coating based on allogeneic collagen and platelet-rich plasma lysate in the surgical treatment of symblepharon. Experimental study

Purpose of the study was to evaluate the efficacy of a combination of allogeneic collagen-based mesh covering and platelet-rich plasma (PRP) lysate for the surgical treatment of symblepharon in an animal experiment.

Material and methods. The study was approved by the local ethics committee of the Helmholtz National Medical Research Center of Eye Diseases (protocol No. 63/1 dated February 16, 2023). Thirty-two mature chinchilla rabbits (64 eyes) were divided into four equal groups based on the time of withdrawal from the experiment. Collagen isolated from rabbit tendons was used to manufacture the mesh covering. Allogeneic plasma-free lysate was obtained from the venous blood of rabbits not included in the study. A 10 × 10 mm defect down to the sclera was created in the bulbar conjunctiva of the superior-inner quadrants of both eyes and covered with a membrane made of a reticular wound dressing based on allogeneic collagen. At the end of the surgery, 0,2 ml of PRP lysate was additionally injected into the conjunctiva of the left eye of all rabbits.

Results. On day 45 of observation, pale pink conjunctiva was visualized in the defect area, indistinguishable from the adjacent tissues in color and density. Morphological evaluation revealed complete restoration of the histoarchitecture of the mucous membrane. The use of PRP lysate contributed to a more favorable course of the postoperative period in the first 14 days after surgery.

Conclusion. The unique fibrillar structure of reticular membranes was shown to stimulate the growth of conjunctival epithelial cells and its phenotypic development.

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