Technologies of creation, evaluation of biocompatibility and safety of the collagen matrix in the bioengineering cell construction

Severe diseases of the anterior eye surface, such as ulcers and erosions of varied genesis, or the consequences of the cornea and conjunctiva burns are important topical issues of ophthalmology. Purpose: to assess the suitability of existing collagen substrates based on types I and II collagen for being used as a matrix for rabbit buccal epithelium cells. Material and methods. The study involved 12 rabbits (24 eyes), on whose intact cornea a contact lens was placed. The lens was covered with a variety of type I collagen which had been obtained from rabbit tendon by acid extraction. For control purposes, a contact lens covered with collagen gel based on type II collagen and designed for healing was placed on the cornea of the fellow eye. Biocompatibility and tolerability of collagen matrixes was assessed. Results. Type I collagen obtained from rabbit tendon had no toxic action on buccal epithelial cells. The types of collagen used were found to be an optimal medium for buccal epithelial cells, which adhere well and can be delivered to the site of corneal damage. Type I collagen, which was produced by acid extraction, had an irritating effect on the rabbit cornea, which is associated with high content of acetic acid. Therefore, the technique of producing type I collagen was modified to achieve an additional reduction of acidity. Conclusion. Type I collagen and collagen gel for corneal healing produced on the basis of type II collagen were shown to be safe and effective when used in bioengineering construction // Russian ophthalmological journal. 2017; 10 (2): 71-7. doi: 10.21516/2072-0076-2017-10-2-71-77.

corneal damage, experimental study, work, buccal epithelium, collagen, bioengineering construction, keratoprotector Applicoll

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